Skip to main content
. 2019 Jun 28;93(14):e00693-19. doi: 10.1128/JVI.00693-19

TABLE 3.

Primers used for amplification of HCV genome fragments

Fragment (size [bp]) Primer sequence (5′–3′)
3′ UTR (236) CGGAATTCAGCGGCACACACTAGGTACA (forward, 9443–9462)
GCTCTAGAACATGATCTGCAGAGAGACC (reverse, 9678–9659)
5′ UTR (340) CGGAATTCACCTGCCCCTAATAGGGGCG (forward, 1–20)
GCTCTAGAGGTGCACGGTCTACGAGACC (reverse, 340–321)
SL1-3 (296) CGGAATTCACCTGCCCCTAATAGGGGCG (forward, 1–20)
GCTCTAGAATCAGGCAGTACCACAAGGC (reverse, 296–277)
SL2-4 (301) CGGAATTCCCCCTGTGAGGAACTACTGT (forward, 40–59)
GCTCTAGAGGTGCACGGTCTACGAGACC (reverse, 340–321)
SL3-4 (241) CGGAATTCGTCGTACAGCCTCCAGGCCC (forward, 100–119)
GCTCTAGAGGTGCACGGTCTACGAGACC (reverse, 340–321)