FIG 5.

wY132A AB dimer has a different tertiary structure than hY132A. (a and b) wY132A AB dimer (PDB accession number 6ECS) is overlaid with hY132A EF dimer (PDB accession number 3KXS; labeled in yellow). The top half of the four α-helix bundles and C termini show a distinct structure shift, which is quantified by plots of Cα displacements against residue numbers (c). (d) PONDR VL-XT predicts that hY132A and wY132A have similar disorder patterns, especially at the C termini where missing density is observed (Fig. 4c and d).