FIG 7.
wCp149 assembly is thermodynamically favored. (a) Assemblies of 10 μM hCp149 and wCp149 were compared. Assembly was initiated by addition of 50 mM NaCl. wCp149 shows increasing assembly as the temperature increased. At all temperatures tested, hCp149 showed no assembly with this relatively low ionic strength. (b) A van’t Hoff plot of wCp149 assembly shows that wCp149 assembly at low-ionic strength has a linear temperature (T) dependence. The values for Kcont (the equilibrium constant [K] per pairwise interaction) were derived from the SEC-measured equilibrium concentrations of capsid and dimer, as previously described (15).