TABLE 2.
Data collection and refinement statistics of wCp149 capsid
Parameter | Value for the parameter |
---|---|
Data collection | |
Microscope | FEI Titan Krios |
Voltage (kV) | 300 |
Detector | Gatan K2 Summit |
Dose (e−/Å2) | 30 |
Pixel size (Å) | 0.86 |
Defocus range (μm) | 0.739–3.888 |
Reconstruction (RELION) | |
No. of micrographs | 251 |
No. of particles | 7911 |
Symmetry | Icosahedron |
Sharpening B-factor (Å2) | −214 |
Final resolution (Å) | 4.52 |
EMDB accession code | EMD-9031 |
Model refinement (PHENIX) | |
CCvolume | 0.855 |
CCmasked | 0.858 |
RMSD bond length (Å) | 0.006 |
RMSD bond angles (°) | 0.871 |
Ramachandran plot | |
Favored (%) | 95.46 |
Allowed (%) | 4.54 |
Outliers (%) | 0.00 |
Clashscore | 3.28 |
Avg B-factor (Å2) | 133.17 |
PDB code | 6EDJ |