TABLE 1.
Reagent or resource | Source | Identifier | Analysis method(s) (dilution)a |
---|---|---|---|
Mouse anti-SV40 T antigen (pAb416) | Abcam | pAb16879 | IFA (1:250), WB (1:2,000) |
Mouse anti-SV40 VP1 (P5G6) | Abcam | pAb53977 | WB (1:2,000) |
Rabbit anti-SV40 VP2/VP3 | Abcam | Ab53983 | WB (1:2,000) |
Rabbit anti-β-actin | Cell Signaling | 4968 | WB (1:2,000) |
Rabbit anti-pChk1Ser10 | Cell Signaling | 2344 | WB (1:500) |
Rabbit anti-pChk2Thr68 | Cell Signaling | 2661 | WB (1:500) |
Rabbit anti-pATMThr1981 | Abcam | GR125270 | WB (1:2,000) |
Mouse anti-pCdk1Tyr15 | Santa Cruz | sc-136014 | WB (1:1,000) |
Rabbit anti-pCdk2Tyr15 | Cell Signaling | 9111 | WB (1:1,000) |
Rabbit anti-pWee1S642 | Cell Signaling | 4910 | WB (1:1,000) |
Mouse anti-Cdk1 | Abcam | Ab18 | WB (1:1,000) |
Mouse anti-Cdk2 | Santa Cruz | sc-6248 | WB (1:1,000) |
Moue anti-Cdc25C | Santa Cruz | sc-7389 | WB (1:1,000) |
Mouse anti-Wee1 | Santa Cruz | sc-5285 | WB (1:1,000) |
Mouse anti-cyclin D1 | Santa Cruz | sc-8396 | WB (1:1,000) |
Mouse anti-cyclin E | Santa Cruz | sc-247 | WB (1:1,000) |
Mouse anti-cyclin A | Santa Cruz | sc-27682 | WB (1:1,000) |
Mouse anti-cyclin B1 | Santa Cruz | sc-245 | WB (1:1,000) |
Alexa Fluor 647-rabbit anti-pH3Ser10 | Cell Signaling | 3458 | FACS 1:100 |
HRP anti-mouse IgG | General Electric | NA931 | WB (1:3,000 to 1:5,000) |
HRP anti-rabbit IgG | General Electric | NA934 | WB (1:3,000 to 1:5,000) |
IRDye 800CW anti-mouse IgG | Li-Cor | 926-332210 | WB (1:10,000 to 1:20,000) |
IRDye 680RD anti-rabbit IgG | Li-Cor | 926-68071 | WB (1:10,000 to 1:20,000) |
Alexa Fluor 594 goat anti-mouse IgG(H + L) secondary antibody | Invitrogen | A11005 | IFA (1:250) |
IFA, immunofluorescence assay; WB, Western blotting; FACS, fluorescence-activated cell sorting.