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. 2019 Jun 28;93(14):e00130-19. doi: 10.1128/JVI.00130-19

TABLE 1.

List of antibodies used for Western analysis, immunofluorescent microscopy analysis, and fluorescence-activated cell sorting

Reagent or resource Source Identifier Analysis method(s) (dilution)a
Mouse anti-SV40 T antigen (pAb416) Abcam pAb16879 IFA (1:250), WB (1:2,000)
Mouse anti-SV40 VP1 (P5G6) Abcam pAb53977 WB (1:2,000)
Rabbit anti-SV40 VP2/VP3 Abcam Ab53983 WB (1:2,000)
Rabbit anti-β-actin Cell Signaling 4968 WB (1:2,000)
Rabbit anti-pChk1Ser10 Cell Signaling 2344 WB (1:500)
Rabbit anti-pChk2Thr68 Cell Signaling 2661 WB (1:500)
Rabbit anti-pATMThr1981 Abcam GR125270 WB (1:2,000)
Mouse anti-pCdk1Tyr15 Santa Cruz sc-136014 WB (1:1,000)
Rabbit anti-pCdk2Tyr15 Cell Signaling 9111 WB (1:1,000)
Rabbit anti-pWee1S642 Cell Signaling 4910 WB (1:1,000)
Mouse anti-Cdk1 Abcam Ab18 WB (1:1,000)
Mouse anti-Cdk2 Santa Cruz sc-6248 WB (1:1,000)
Moue anti-Cdc25C Santa Cruz sc-7389 WB (1:1,000)
Mouse anti-Wee1 Santa Cruz sc-5285 WB (1:1,000)
Mouse anti-cyclin D1 Santa Cruz sc-8396 WB (1:1,000)
Mouse anti-cyclin E Santa Cruz sc-247 WB (1:1,000)
Mouse anti-cyclin A Santa Cruz sc-27682 WB (1:1,000)
Mouse anti-cyclin B1 Santa Cruz sc-245 WB (1:1,000)
Alexa Fluor 647-rabbit anti-pH3Ser10 Cell Signaling 3458 FACS 1:100
HRP anti-mouse IgG General Electric NA931 WB (1:3,000 to 1:5,000)
HRP anti-rabbit IgG General Electric NA934 WB (1:3,000 to 1:5,000)
IRDye 800CW anti-mouse IgG Li-Cor 926-332210 WB (1:10,000 to 1:20,000)
IRDye 680RD anti-rabbit IgG Li-Cor 926-68071 WB (1:10,000 to 1:20,000)
Alexa Fluor 594 goat anti-mouse IgG(H + L) secondary antibody Invitrogen A11005 IFA (1:250)
a

IFA, immunofluorescence assay; WB, Western blotting; FACS, fluorescence-activated cell sorting.