TABLE 1.
Biochemical phenotypes of wild-type and mutant polymerasesa
| 3Dpol mutation(s) | RNA initiation (min) | EC stability (min) | Processive elongation (nt/sec) | Processive Km value (μM) |
|---|---|---|---|---|
| WT | 6 ± 1 | 88 ± 9 | 22 ± 1 | 67 ± 2 |
| D79H | 5 ± 1 | 70 ± 30 | 21 ± 1 | 64 ± 3 |
| G64S | 8 ± 1 | 24 ± 7 | 12 ± 1 | 46 ± 4 |
| L420A | 8 ± 1 | 28 ± 4 | 30 ± 1 | 75 ± 3 |
| G64S and L420A | 6 ± 3 | 40 ± 8 | 26 ± 1 | 84 ± 9 |
a
Single-nucleotide incorporation and processive elongation were assayed in 75 mM NaCl (pH 7) at 30°C. Values are ± standard errors of parameters from curve fitting. EC, elongation complex.