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Effects of the D286R mutation in UL31 on its interaction with UL34 in HSV-1-infected cells. Vero cells were mock infected or infected with wild-type HSV-1(F), YK726 (UL31-D286R), or YK722 (ΔUL34) at an MOI of 3 for 18 h, harvested, immunoprecipitated (IP) with anti-UL34 or anti-UL50 antibody, and analyzed by immunoblotting with anti-UL34, anti-UL31, and anti-UL47 antibodies. The numbers to the left of the gels are molecular masses (in kilodaltons).
