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Effects of the D286R mutation in UL31 on viral growth in HeLa cells. HeLa cells were infected with wild-type HSV-1(F), YK726 (UL31-D286R), or YK727 (UL31-D286R-repair) at an MOI of 10 (A) or 0.05 (B), harvested at the indicated times postinfection, and assayed on Vero cells. Each data point is the mean ± standard error from 3 independent experiments. *, P < 0.05 (Tukey’s test) for YK726 (UL31-D286R) versus HSV-1(F) and YK726 (UL31-D286R) versus YK727 (UL31-D286R-repair).
