FIG 9.

Effects of the D286R mutation in UL31 on the subcellular localization of UL31 and UL34 in HSV-1-infected Vero cells. (A and B) Vero cells were infected with wild-type HSV-1(F), YK726 (UL31-D286R), or YK727 (UL31-D286R-repair) at an MOI of 3, fixed at 18 h postinfection, permeabilized, stained with anti-UL31 and anti-UL34 antibodies (A) or anti-UL34 and lamin A/C antibodies (B), and examined by confocal microscopy. Bars, 10 μm. (C) Percentage of cells (of 30 to 40 cells in each experiment) showing a nuclear rim localization of UL34 (nuclear rim), needle-like structures of UL34 (needle), and other punctate structures of UL34 (other) in the experiment whose results are presented in panel B. Data are shown as the mean ± SEM from 3 independent experiments.