TABLE 5.
Effect of the UL31-D286R mutation on the distribution of virus particles in infected Vero cells
| Virus | Mean % of virus particles in each morphogenetic stage ± SEa |
Total no. of particles/total no. of cells counted | ||||
|---|---|---|---|---|---|---|
| Nucleocapsids in nucleus | Enveloped virions in perinuclear space | Nucleocapsids in cytoplasm | Enveloped virions in cytoplasm | Extracellular enveloped virions | ||
| HSV-1(F) | 55.6 ± 9.0 (633) | 6.2 ± 2.4 (71) | 12.0 ± 4.3 (136) | 3.1 ± 0.8 (35) | 23.1 ± 3.5 (263) | 1,138/12 |
| YK726 (UL31-D286R) | 96.5 ± 18.3 (626) | 0 (0) | 1.5 ± 0.6 (10) | 0.6 ± 0.3 (4) | 1.4 ± 0.6 (9) | 649/12 |
| YK727 (UL31-D286R-repair) | 58.2 ± 8.8 (899) | 6.0 ± 2.3 (92) | 9.1 ± 2.6 (140) | 3.2 ± 0.9 (50) | 23.6 ± 3.0 (364) | 1,545/12 |
a
Numbers in parentheses are the numbers of virus particles.