TABLE 6.
Effect of UL31-D286R mutation on the distribution of virus particles in infected HeLa cells
| Virus | Mean % of virus particles in each morphogenetic stage ± SEa |
Total no. of particles/total no. of cells counted | ||||
|---|---|---|---|---|---|---|
| Nucleocapsids in nucleus | Enveloped virions in perinuclear space | Nucleocapsids in cytoplasm | Enveloped virions in cytoplasm | Extracellular enveloped virions | ||
| HSV-1(F) | 54.7 ± 10.5 (690) | 4.3 ± 2.0 (54) | 14.0 ± 3.6 (177) | 4.4 ± 1.5 (56) | 22.6 ± 4.3 (285) | 1,262/12 |
| YK726 (UL31-D286R) | 92.7 ± 18.8 (758) | 0 (0) | 4.8 ± 1.5 (39) | 0.5 ± 0.3 (4) | 2.1 ± 1.1 (17) | 818/12 |
| YK727 (UL31-D286R-repair) | 52.6 ± 12.1 (687) | 4.7 ± 2.3 (62) | 10.4 ± 2.4 (136) | 4.1 ± 1.6 (53) | 28.2 ± 7.6 (368) | 1,306/12 |
a
Numbers in parentheses are the numbers of virus particles.