FIG 7.

Rabbit and NHP antibody responses to both prime/boost regimens were similar. New Zealand White rabbits and rhesus macaques received a priming immunization of MVAgp120 in week 0 and gp120 boosts in weeks 12 and 16. All the vaccines were either administered intramuscularly (i.m. prime/i.m. boost) or the MVAgp120 prime was administered intranasally and boosting consisted of both intramuscular and intranasal components (i.n./i.m.-plus-i.n.) (n = 6/group). The animals were bled at the time points indicated, and antibody responses were determined by ELISA. (A) Serum gp120-specific IgG responses after administration of the MVAgp120 prime (weeks 6 to 8), the first gp120 boost (week 15), and the second gp120 boost (week 19). The connecting lines and error bars represent the geometric mean titers and the 95% confidence intervals. (B) After administration of the second booster vaccine (week 19), rabbits and NHPs had similar serum V1V2-specific IgG and serum gp120-specific IgA responses. Fecal gp120-specific responses were also similar between the species. The horizontal lines represent the geometric mean titers.