. 2019 Jun 28;93(14):e00475-19. doi: 10.1128/JVI.00475-19

TABLE 2.

Animal characteristics and vaccine specifications for optimization experiments in mice and rabbits

Expt	Species	Supplier	Sex	Bleed days	Vaccination days	Vaccination route	Antigen	Adjuvant	Total vaccine vol (μl)	μl/nostril if IN; μl/leg if IM
Adjuvants	C57BL/6 mice stock no. 000664	Jackson Labs	Female	−4, 14, 35	0, 7, 21	i.n.	10 μg gp120	None; 7 μg M7-NH2; 15 μg C48/80; 1 μg CT	15	7.5
Adjuvants	C57BL/6 mice stock no. 000664	Jackson Labs	Female	−4, 14, 35	0, 7, 21	i.n.	10 μg gp120	10 μg MPL	21	10.5
IN 1	NZW rabbits^a	Robinson	Male	0, 21, 42	0, 28	i.m.	100 μg gp120	Addavax	400	200
IN 1	NZW rabbits^a	Robinson	Male	0, 21, 42	0, 28	i.n.	100 μg gp120, 200 μg gp120, 300 μg gp120	64 μg M7-NH2	340	85; 20-s rest^b
IN 2	NZW rabbits	Robinson	Male	−6, 21, 48	0, 34	i.m.	100 μg gp120	Addavax	400	200
IN 2	NZW rabbits	Robinson	Male	−6, 21, 48	0, 34	i.n.	300 μg gp120; 145 μg gp120-Ad2F; 290 μg gp120-Ad2F; 435 μg gp120-Ad2F	64 μg M7-NH2	440	73.33; 15-s rest
IN 3	NZW rabbits	Covance	Male	−15, 21, 42	0, 28	i.m.	100 μg gp120	Addavax	400	200
IN 3	NZW rabbits	Covance	Male	−15, 21, 42	0, 28	i.n.	300 μg gp120; 145 μg gp120-Ad2F; 435 μg gp120-Ad2F	64 μg M7-NH2	440	73.33; 15-s rest
MVA 1	NZW rabbits	Robinson	Female	0, 21, 35	0, 21	i.m.	1 × 10⁸ PFU MVAgp120, 5 × 10⁶ PFU MVAgp120; 1 × 10⁸ PFU MVAdelta5gp120, 5 × 10⁶ PFU MVAdelta5gp120		200	200
MVA 2	NZW rabbits	Covance	Female	0, 21, 35	0, 21	i.m.	1 × 10⁸ PFU MVAgp120, 1 × 10⁸ PFU MVAdelta5gp120, 1 × 10⁹ PFU MVAdelta5gp120, 1 × 10¹⁰ PFU MVAdelta5gp120		230	230
MVA 2	NZW rabbits	Covance	Female	0, 21, 35	0, 21	i.n.	1 × 10⁹ PFU MVAdelta5gp120		200	50; 30-s rest
MVA 3	NZW rabbits	Robinson	Female	0, 21, 35	0, 28	i.m.	1 × 10⁸ PFU MVAgp120		200
						i.n.	3 × 10⁸ PFU MVAgp120		200	50; 30-s rest
						Rectal	3 × 10⁸ PFU MVAgp120		200
						Gastric	3 × 10⁸ PFU MVAgp120		1,000

NZW rabbits, New Zealand White rabbits.

rest, number of seconds the animal is rested after an aliquot of the vaccine was delivered to both the left and right nostril prior to receiving another aliquot of the vaccine. The animal was maintained in dorsal recumbency with isoflurane administered as needed to maintain surgical anesthesia for the indicated number of seconds prior to receiving another aliquot of vaccine to each nostril or being placed in sternal recumbency for recovery.