TABLE 3.
Animal characteristics and vaccine specifications for prime/boost experiments
| Expt | Species | Sex | Supplier | Bleed wks | Prime |
Boost |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Wk | Route | MVAgp120 amt (PFU) | Wks | Route | Antigen | Adjuvant | |||||
| Prime/boost 1 | NZW rabbits | Both | Robinson | 0, 6, 15, 19 | 0 | i.m. | 1 × 108 | 12, 16 | i.m. | 100 μg gp120 | Addavax |
| NZW rabbits | Female | Robinson | 0, 6, 15, 19 | 0 | i.m. | 1 × 108 | 12, 16 | i.n. | 435 μg gp120-Ad2F | 64 μg M7-NH2 | |
| NZW rabbits | Female | Robinson | 0, 6, 15, 19 | 0 | i.m. | 1 × 108 | 12, 16 | i.m. + i.n. | 100 μg gp120 (i.m.); 435 μg gp120-Ad2F (i.n.) | Addavax (i.m.); 64 μg M7-NH2 (i.n.) | |
| NZW rabbits | Both | Robinson | 0, 6, 15, 19 | 0 | i.n. | 3 × 108 | 12, 16 | i.m. + i.n. | 100 μg gp120 (i.m.); 435 μg gp120-Ad2F (i.n.) | Addavax (i.m.); 64 μg M7-NH2 (i.n.) | |
| Prime/boost 2 | Rhesus macaques | Both | New England Primate Research Center | −2, 8, 15, 19, 23, 27 | 0 | i.m. | 1 × 108 | 12, 16, 24 | i.m. | 100 μg gp120 | Addavax |
| Rhesus macaques | Both | New England Primate Research Center | −2, 8, 15, 19, 23, 27 | 0 | i.n. | 3 × 108 | 12, 16, 24 | i.m. + i.n. | 100 μg gp120 (i.m.); 435 μg gp120-Ad2F (i.n.) | Addavax (i.m.); 64 μg M7-NH2 (i.n.) | |