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. 2019 Jun 28;93(14):e00544-19. doi: 10.1128/JVI.00544-19

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FIG 4 — NL and AD8 Env interaction with Ser5. (A) Indicated Ser5-Env BiFC pairs were expressed in 293T cells and analyzed by flow cytometry. The NL and AD8 Env interactions with Ser5 were then calculated and presented as relative values, with the levels of the Ser5-VN/NL-VC pair set as 100%. (B) 293T cells were transfected with 1 μg pH22ΔN or pH-AD8ΔN in the presence of 25 ng pCMV6-Ser2-FLAG or pCMV6-Ser5-FLAG. Proteins were pulled down by anti-FLAG and analyzed by Western blotting. Pulled-down Env proteins were quantified by ImageJ, and their relative values are shown on the bottom, with the levels of NL Env set as 100%. (A and B) Error bars represent SDs of the results from three independent experiments. **, P < 0.01.