FIG 6.

Dissociation of AD8 Env trimers by Ser5 in the presence of CD4. NL and AD8 Env BiFC fusion proteins were expressed pairwise in 293T cells with increasing amounts of pBJ5-Ser5-HA or pBJ6-Ser2-HA in the presence or absence of 1 μg pCMV6-CD4-FLAG. Levels of Env-Env association were detected by flow cytometry and calculated, with the levels in the absence of SERINC proteins set as 100%. Error bars represent SDs of the results from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.