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. 2019 Jun 28;93(14):e00544-19. doi: 10.1128/JVI.00544-19

FIG 8.

FIG 8

Inhibition of tier 2/3 virus replication by Ser5 in the presence of CD4. (A) NL-ΔN and AD8-ΔN viruses were produced from 293T cells in the presence of indicated amounts of pCMV6-CD4-FLAG and pCMV6-Ser5-FLAG. Viral infectivity was determined in TZM-bI cells and presented as relative values, with the infectivity of viruses produced in the absence of Ser5 set as 100%. (B) Indicated viruses were produced from 293T cells in the presence or absence of 50 ng pCMV6-Ser5-FLAG with or without 1 μg pCMV6-CD4-FLAG. Viral infectivity was determined in TZM-bI cells and presented as relative values, with the infectivity in the absence of Ser5 set as 100%. Error bars indicate SDs of the results from three experiments.