FIG 2.

C18 MAbs targeted six distinct antigenic sites. Hierarchical clustering of 13 C18 MAbs (in bold type) and 13 reference MAbs based on Ward’s minimal variance analysis of MAb binding to a library of strain H77 E1E2 proteins with single alanine mutations spanning the full E1E2 sequence, depicted as an unrooted tree. Bootstrap values greater than 80 are shown. MAbs that clustered together with bootstrap values of >90 were highlighted in the same color and given a label indicating a previously described (AR1, AR2, AR3, and AR4-5) or novel (AS108, AS112, and AS146) antigenic site.