FIG 7.

Polymorphisms outside MAb binding sites influence bNAb resistance. Amino acids present at probable binding residues of HEPC111 (A), HEPC153 (B), and HEPC151-1 (C) across all strains in the HCVpp panel. Homology to the strain H77 sequence is indicated with a dot. Percent neutralization of each HCVpp strain by 50 μg/ml of each MAb is shown. Values are averages of two independent experiments, each performed in duplicate. For each MAb, full neutralization curves were also measured in duplicate with 5-fold serial dilutions of MAb starting at 100 μg/ml for one sensitive and two more resistant HCVpp strains. Error bars indicate standard deviations. MAb names are color coded according to hierarchical clustering in Fig. 2.