FIG 8.

C18 MAbs formed three functional groups based on neutralization profiles. Neutralization of 18 HCVpps by 50 μg/ml of each MAb was measured in duplicate, generating a neutralization profile for each MAb (i.e., a ranking of HCVpp strains by relative neutralization sensitivity). Pearson’s correlations were measured between these C18 MAb neutralization profiles and neutralization profiles of reference MAbs. (A) Neutralization testing of reference MAb HEPC74 was performed in parallel with testing of C18 MAbs. Correlation was measured between the neutralization profile of this repeat test of HEPC74 (HEPC74-test) and the previously published HEPC74 neutralization profile (HEPC74-reference, left) or an unrelated MAb (AR5A-reference, right). Each point indicates a single HCVpp, with rank of neutralization sensitivity to one MAb on the x axis and another MAb on the y axis. (B) Pearson r values of pairwise correlations between neutralization profiles of each C18 MAb (y axis) and each reference MAb (x axis). Only r values that are statistically significant (P < 0.05) are shown, and darker green color indicates a stronger positive correlation. The highest r value for each C18 MAb is boxed and in bold type. C18 MAbs clustered into three functional groups, namely, AR3-like, AR1-like, and AR5-like.