TABLE 3.
Germ line origin genes and variable region analysis of subject C18 MAbs
| Antigenic site | MAb | Heavy chain variable gene sequence |
Light chain variable sequence |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| V gene and allele | V region nucleotide homology to V-gene(%) | D gene and allele | J gene and allele | CDR3 amino acids | CDR3 length (aa)a | V gene and allele | V region nucleotide homology to V gene (%) | J gene and allele | CDR3 amino acids | CDR3 length (aa) | ||
| AR1 | HEPC-167 | IGHV1-18*04 | 91.7 | IGHD3-16*01 | IGHJ4*03 | ARDSVTGGMDV | 11 | IGLV3-25*03 | 92.8 | IGLJ3*02 | QFADISGTYRRV | 12 |
| AR3 | HEPC-122 | IGHV1-69*17 | 93.8 | IGHD3-3*01 | IGHJ4*02 | ATFDYDFYSGAPSRSYFVH | 19 | IGKV3-20*01 | 97.2 | IGKJ4*01 | QQYGSSPT | 8 |
| HEPC-151-1 | IGHV1-69*01 | 87.9 | IGHD3-10*01 | IGHJ6*02 | ARDGGGHSGLGRFYDLNGMDV | 21 | IGKV3-15*01 | 95.3 | IGKJ2*01 | QQYDNWPPRYT | 11 | |
| HEPC-153 | IGHV1-69*01 | 92.7 | IGHD1-20*01 | IGHJ5*02 | VREMRYNWNPGRSSWFDP | 18 | IGKV3-11*01 | 93.6 | IGKJ3*01 | QQRSIRPPS | 9 | |
| HEPC-154 | IGHV3-53*01 | 88.8 | IGHD3-16*01 | IGHJ3*02 | ARCFAGLMAMDI | 12 | IGKV2-28*01 | 97.3 | IGKJ3*01 | MQGLQIPPT | 9 | |
| AR4-5 | HEPC-111 | IGHV1-69*01 | 91.7 | IGHD3-3*01 | IGHJ3*01 | ARSEDVLSGYYSVSIDAFNV | 20 | IGLV1-51*01 | 94.0 | IGLJ2*01 | ATWDRSLSIAV | 11 |
| HEPC-130 | IGHV3-30*03 | 87.2 | IGHD2-2*01 | IGHJ6*02 | ARNSVPATMTSYYFYALDL | 19 | IGKV1-39*01 | 88.9 | IGKJ1*01 | LQTYDTVWT | 9 | |
| AS108 | HEPC-108 | IGHV1-69*06 | 94.1 | IGHD6-19*01 | IGHJ4*02 | ARASIVNISGFVRFRFDS | 18 | IGKV1-33*01 | 95.7 | IGKJ2*01 | QHYDDVPPVYT | 11 |
| HEPC-151-2 | IGHV1-18*04 | 89.6 | IGHD3-22*01 | IGHJ3*02 | ARHDSSGYEALDI | 13 | IGLV3-19*01 | 97.9 | IGLJ1*01 | NSRDTSPYHHFV | 12 | |
| HEPC-132 | IGHV4-31*03 | 91.8 | IGHD3-3*01 | IGHJ6*02 | ARDGADGFWSGHNFGMDA | 18 | IGKV3-11*01 | 93.6 | IGKJ4*01 | QQRSAWPRT | 9 | |
| HEPC-158 | IGHV1-18*04 | 89.6 | IGHD3-22*01 | IGHJ3*02 | ARHDSSGYEALDI | 13 | IGLV3-19*01 | 97.9 | IGLJ1*01 | NSRDTSPYHHFV | 12 | |
| AS146 | HEPC-146 | IGHV4-61*01 | 92.1 | IGHD5-24*01 | IGHJ1*01 | ARTSQMTTLLL | 11 | IGLV2-8*01 | 94.4 | IGLJ3*02 | SSYVGNDNLL | 10 |
| AS112 | HEPC-112 | IGHV3-48*03 | 92.4 | IGHD3-3*01 | IGHJ4*02 | VRDGREFWGGSRIPFDH | 17 | IGKV3-20*01 | 95.0 | IGKJ2*01 | QQYATTPDT | 9 |
a
aa, amino acid.