FIG 1.

3p10LG9 induces an interferon response and is effective as prophylaxis against DENV-2 infection in both U937-DC-SIGN cells and A549 cells. (A) Structure and sequence of 3p10L and 3p10LG9. (B) 3p10L, 3p10LG9, or G9neg was transfected into U937-DC-SIGN or A549 cells. Supernatants were harvested and incubated on ISRE-luc HEK-293T reporter cells. Luminescence was measured 6 h after incubation with the supernatant. Tables show EC₅₀ values (nM) for 3p10L and 3p10LG9. (C) Transfected cells were infected with DENV-2 (TSV01) at an MOI of 1 and stained with antibodies binding NS1 and the E protein (4G2) at 24 h after infection. Tables show EC₅₀ values (nM) for 3p10L and 3p10LG9. (D) Representative flow cytometry graphs for infected U937-DC-SIGN cells (left) or A549 cells (right). For U937-DC experiments, values are means ± standard errors of the means (SEM) (n = 6) from two independent experiments. For A549 experiments, values are means ± SEM (n = 4) from two independent experiments. The dotted lines show the mean for the G9neg control. Statistical significance for the luciferase assay and infection assay in panels B and C was calculated for U937-DC-SIGN cells (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001) and A549 cells (**, P ≤ 0.01; ****, P ≤ 0.0001), using ordinary two-way analysis of variance (ANOVA) with Sidak’s multiple-comparison test.