FIG 6.

Characterization of eCD4-Ig-induced residue changes with monoclonal and polyclonal antibodies. TZM-bl cells were incubated with HIV-1 pseudoviruses pseudotyped with Env of the SHIV-SF162P3 sequence or variants including eCD4-Ig-selected substitutions, in the presence of the indicated concentrations of the neutralizing antibodies N6 (CD4-binding site), 10-1074 (V3 glycan), 447-52D (V3 loop), or E51 (CD4-induced) or with serum from an HIV-1-positive person. Results are representative of two independent experiments. Error bars represent SEM. Note that V3 loop substitutions increased sensitivity to 447-52D and E51 and variants with the Q428K substitution were more susceptible to serum neutralization.