Table 3.
Gene | Primers Sequence | Conditions | Product Size (bp) |
---|---|---|---|
IL-1α | 5’-CATGTCAAATTTCACTGCTTCATCC -3’ 5’-GTCTCTGAATCAGAAATCCTTCTATC -3’ |
5 s at 95 °C, 8 s at 55 °C, 17 s at 72 °C for 45 cycles |
421 |
TNF-α | 5’-CAGAGGGAAGAGTTCCCCAG -3’ 5’-CCTTGGTCTGGTAGGAGACG -3’ |
5 s at 95°C, 6 s at 57°C, 13 s at 72°C for 40 cycles |
324 |
IL-6 | 5’-ATGAACTCCTTCTCCACAAGCGC-3’ 5’-GAAGAGCCCTCAGGCTGGACTG-3’ |
5 s at 95°C, 13 s at 56°C, 25 s at 72°C for 40 cycles |
628 |
IL-8 | 5-ATGACTTCCAAGCTGGCCGTG -3’ 5-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3’ |
5 s at 94°C, 6 s at 55°C, 12 s at 72°C for 40 cycles |
297 |
TGF-β | 5’-CCGACTACTACGCCAAGGAGGTCAC-3’ 5’-AGGCCGGTTCATGCCATGAATGGTG-3’ |
5 s at 94°C, 9 s at 60°C, 18 s at 72°C for 40 cycles |
439 |
IL-1β | 5’-GCATCCAGCTACGAATCTCC-3’ 5’-CCACATTCAGCACAGGACTC-3’ |
5 s at 95°C, 14 s at 58°C, 28 s at 72°C for 40 cycles |
708 |