Table 3.

Primer sequences and RT-PCR conditions.

Gene	Primers Sequence	Conditions	Product Size (bp)
IL-1α	5’-CATGTCAAATTTCACTGCTTCATCC -3’ 5’-GTCTCTGAATCAGAAATCCTTCTATC -3’	5 s at 95 °C, 8 s at 55 °C, 17 s at 72 °C for 45 cycles	421
TNF-α	5’-CAGAGGGAAGAGTTCCCCAG -3’ 5’-CCTTGGTCTGGTAGGAGACG -3’	5 s at 95°C, 6 s at 57°C, 13 s at 72°C for 40 cycles	324
IL-6	5’-ATGAACTCCTTCTCCACAAGCGC-3’ 5’-GAAGAGCCCTCAGGCTGGACTG-3’	5 s at 95°C, 13 s at 56°C, 25 s at 72°C for 40 cycles	628
IL-8	5-ATGACTTCCAAGCTGGCCGTG -3’ 5-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3’	5 s at 94°C, 6 s at 55°C, 12 s at 72°C for 40 cycles	297
TGF-β	5’-CCGACTACTACGCCAAGGAGGTCAC-3’ 5’-AGGCCGGTTCATGCCATGAATGGTG-3’	5 s at 94°C, 9 s at 60°C, 18 s at 72°C for 40 cycles	439
IL-1β	5’-GCATCCAGCTACGAATCTCC-3’ 5’-CCACATTCAGCACAGGACTC-3’	5 s at 95°C, 14 s at 58°C, 28 s at 72°C for 40 cycles	708