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. 2019 Jun 9;20(11):2815. doi: 10.3390/ijms20112815

Figure 3.

Figure 3

Relative expression levels of CsWRKY7 in tea plants (A) Tissue expression profiles of CsWRKY7 in ‘Longjing 43’. Different tissues include bud, 1st leaf, 2nd leaf, 3rd leaf, old leaf, flower, stem, and root. The expression levels of CsWRKY7 in different tissues were compared with the bud. (B) The transcript levels of CsWRKY7 under cold (4 °C) and heat (38 °C). CsWRKY7 expression levels are detected at four different time points (0, 4, 8 and 12 h) post-temperature stress treatment. (C) Relative expression levels of CsWRKY7 under different abiotic stress. Two-year-old tea seedlings were treated with 150 mM NaCl, 90 mM sucrose (Suc), 10% (w/v) PEG4000 (PEG) and 90 mM mannitol (Man), and samples were harvested at the time intervals indicated. (D) The transcript levels of CsWRKY7 under various phytohormone, including 100 μM indolyl-3-acetic acid (IAA), 5 μM naphthalene-1-acetic acid (NAA), 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 100 μM abscisic acid (ABA), 50 μM methyl jasmonate (MeJA), 5 mM salicylic acid (SA), and 100 μM gibberellins (GA), which were added to the culture solution, the functional leaves were harvested at 0, 3, 6, or 24 h post-treatment. Treated samples at 0 h served as controls. Error bars represent ± S.E. for three independent experiments. The significant level is presented by the asterisks (* p < 0.05, ** p < 0.01).