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The demethylating agent decitabine (DAC) stimulates hsa-miR-136-5p expression and represses ERα36 one. MCF-7 cells were treated for 48 h with DMSO 0.01% (as control) or the demethylating agent DAC 5 µM. (a) hsa-miR-136-5p expression level was assessed using TaqMan™ microRNA assay and (b) ERα36 expression was measured by RT-qPCR analysis. Each bar represents mean ± S.E.M. (a) N = 6 (b) N ≥ 3. * P < 0.05; ** P < 0.01.
