Figure 5.

ERα36 expression is related to the methylation status of its promoter in breast tumor samples. Seventeen breast tumor samples were collected from the tumor biobank of the Cancerology Institute of Lorraine. ERα36 promoter genomic DNA was submitted to bisulfite sequencing and ERα36 expression level was performed by real-time PCR analyses in triplicates for each tumor sample. The correlation between each CpG methylation and ERα36 expression was established by a MATLAB program (Pearson rank correlation test). * CpG which methylation level is significantly (P < 0.05) correlated to ERα36 expression level.