Skip to main content
. 2019 May 29;20(11):2637. doi: 10.3390/ijms20112637

Figure 6.

Figure 6

Decitabine directly targets ERα36 genomic sequence. (a) MCF-7 cells were treated for 48 h with DMSO (as control), DAC or ICI 182,780 (N = 4); (b) MCF-7 cells transfected with either scrambled siRNA (si control, N = 4) or siRNA targeting the exon 1 of ESR1 (siERα66, N = 3) were treated for 48 h with DMSO (as control) or DAC. The methylation level of the ERα36 promoter fourth CpG island was assessed after bisulfite conversion. A percentage of methylation level variation was calculated for DAC or ICI treated cells versus DMSO ones. (c) Representative Western blot analysis of ERα66 expression in MCF-7 cells transfected with either si control or si ERα66. α-Tubulin was used as a loading control. Each bar represents mean ± S.E.M. ** P < 0.01.