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Normalized melting curves analysed by: a) Precision Melt Analysis software (40), and b) LightCycler® 96 software (39). PCR was performed using LightCycler 480 HRM MasterMix. Samples: 1=1-103 dilutions of Lactobacillus curvatus, 2=104 dilution of L. curvatus, 3=1-103 dilutions of L. sakei, and 4=104 dilution of L. sakei. RFU=relative fluorescence unit
