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. 2019 Mar;57(1):97–104. doi: 10.17113/ftb.57.01.19.5983

Table S1. Melting temperature (T_m) and threshold cycle values of positive controls and their dilutions obtained by CFX96 (40).

Lactobacillus sakei				Lactobacillus curvatus
Dilution	T_m/°C	Threshold cycle		Dilution	T_m/°C	Threshold cycle
LightCycler 480 HRM master kit
1	(78.5±0.1)^a	(21.7±0.2)^a		1	(79.1±0.1)^a	(21.55±0.07)^a
10^-1	(78.4±0.0)^a	(25.09±0.03)^a		10^-1	(79.0±0.0)^a	(25.23±0.08)^a
10^-2	(78.4±0.0)^a	(28.47±0.09)^a		10^-2	(79.1±0.1)^a	(28.52±0.08)^a
10^-3	(78.4±0.0)^a	(32.2±0.2)^a		10^-3	(79.0±0.0)^a	(32.32±0.03)^a
10^-4	(78.2±0.0)^a	(35.9±0.6)^a		10^-4	(79.0±0.0)^a	(35.8±0.6)^a
Total	(78.4±0.1)^b			Total	(80.45±0.03)^b
Real-time PCR reagent
1	(79.4±0.0)^a	(20.3±0.1)^a		1	(80.0±0.0)^a	(20.21±0.04)^a
10^-1	(79.3±0.1)^a	(24.07±0.08)^a		10^-1	(79.8±0.0)^a	(24.15±0.05)^a
10^-2	(79.2±0.0)^a	(28.5±0.1)^a		10^-2	(79.8±0.0)^a	(27.8±0.1)^a
10^-3	(79.2±0.0)^a	(31.6±0.3)^a		10^-3	(79.8±0.0)^a	(31.15±0.04)^a
10^-4	(79.2±0.0)^a	(35.3±0.2)^a		10^-4	(79.8±0.0)^a	(34.5±0.4)^a
Total	(79.25±0.09)^b			Total	(79.84±0.08)^b

Data represent the mean value±standard deviation (^aN=3, ^bN=15). PCR=polymerase chain reaction