Figure 3.

CART123 co-cultured with myeloid leukemia cells upregulated CD123 on HUVECs in the in vitro co-culture model. (A) Schematic of the in vitro co-culture model. (B) Effector cells (CART123 or NT) and target cells (MOLM-13, K562, and AML-2) were seeded in the upper chamber, and HUVECs were seeded in the lower chamber of the in vitro co-culture model. After co-culturing for 36 hrs, HUVECs were digested with trypsin to obtain single cell suspension to assess the expression of CD123 by flow cytometry (mean±SEM). Data show the results of six independent experiments. (C) Also, the culture supernatants were harvested and used for analyzing cytokine production by ELISA. (mean±SEM of triplicate). *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Abbreviations: CAR, chimeric antigen receptor; NT, non-transduced T; HUVECs, human umbilical vein endothelial cells; AML, acute myeloid leukemia.