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. 2019 May 31;20(11):2680. doi: 10.3390/ijms20112680

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Artemisinin stimulated the phosphorylation of AMP-activated protein kinase (AMPK) in SH-SY5Y cells. (A,C) The SH-SY5Y cells were collected with artemisinin treatment for different times (0, 15, 30, 60, 90 and 120 min) at 12.5 μM, and at different concentrations (3.15, 6.25, 12.5, 25 and 50 μM) for 120 min. The expression of phosphocreatine AMPK, total AMPK and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were detected by western blot. (B,D) Quantification of representative protein band from western blotting. The data were represented as the mean ± SD. * p < 0.05, ** p < 0.01, versus the control group was considered significantly different.