Figure 6.

Artemisinin attenuated the decrease in cell viability caused by H₂O₂ in neuronal cells. (A) The cytotoxicity of H₂O₂ on neuronal cells. (B) Dose of artemisinin. (C) Primary cultured hippocampal neurons were pretreated with artemisinin at indicated concentrations and then induced with or without 100 μM H₂O₂ for another 24 h, and cell viability was measured using the MTT assay. (D) Primary cultured hippocampal neurons pretreated with 2.5 μM compound C for 30 min were treated with 25 μM artemisinin for 2 h, and then incubated with or without 100 μM H₂O₂ for another 24 h. Immunocytochemistry of NeuN in each group was detected. The pictures were taken at a magnification of 40× (100 μm). (E) Primary cultured hippocampal neurons pretreated with 2.5 μM compound C for 30 min were treated with 25 μM artemisinin for 2 h, and then incubated with or without 100 μM H₂O₂ for another 24 h. Cell viability was measured by the MTT assay. (F) The expression of phosphorylated AMPK, total AMPK, cleaved caspase-3 and GAPDH were detected by western blot. (G,H) Quantitative analysis of (F). The data were represented as the mean ± SD. ** p < 0.01, *** p < 0.001, ^## p < 0.01, ^### p < 0.001.