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. 2019 May 28;20(11):2613. doi: 10.3390/ijms20112613

Figure 2.

Figure 2

Cytoskeleton dynamics is altered in citrate-resistant PC3 cells. (left column) Morphological features of PC3 cells wt, Cit20, and Cit20 WD have been analyzed. Cells were observed with Axiovert 25 (Carl Zeiss, Jena, Germany), and representative pictures were taken with Canon GC5 (Canon Italia S.p.A, 20063 Cernusco sul Naviglio, Milan, Italy) (final magnification 40×). (right column) Actin and the microtubule network were labeled using TRITC-conjugated phalloidin, and a specific anti-αtubulin antibody revealed with alexa-488 conjugated secondary antibody, respectively. Nuclei were stained with DRAQ5. Representative images at low (scale bars 10 μm) and higher magnification (rightmost column, scale bars 5 μm) are shown.