Skip to main content
. 2019 Jun 7;20(11):2792. doi: 10.3390/ijms20112792

Figure 6.

Figure 6

Fibronectin increased cell growth and migration in Caki-1 cells. (A) Caki-1 cells were seeded onto fibronectin (0 and 50 μg/mL)-coated 96-well plates. Twenty-four hours later, cell growth was measured by MTS reduction assay. (B) Caki-1 cells were seeded onto fibronectin (0 and 50 μg/mL)-coated Transwell inserts and subjected to Transwell migration assay for 24 h. The lower chambers were filled with DMEM containing 10% FBS. (C) Caki-1 cells were first incubated with indicated IgG (5 μg/mL) for 30 min before seeding to the Transwell inserts for migration assay (24 h). The lower chambers were filled with DMEM containing fibronectin (0 or 50 μg/mL). (D) Caki-1 cells were seeded onto fibronectin (0 and 50 μg/mL)-coated 24-well plates. Twenty-four hours later, the supernatants were collected and subjected to ELISA for the measurement of TGF-β1. (E) Caki-1 cells were seeded onto fibronectin (0 and 50 μg/mL)-coated six-well plates for 5 h. Proteins were extracted and subjected to Western blot analysis with indicated antibodies. Representative blots are shown. Bar graphs show quantitative results among groups and the value in uncoated group was defined as 100% (A–C, and E). * p < 0.05 vs. uncoated control and # p < 0.05 vs. fibronectin (50 μg/mL) untreated control, n = 3.