Figure 8.

Hypoxia increased RCC cell migration. (A) 786-O cells were seeded onto Transwell inserts and subjected to Transwell migration assay in a normoxia or hypoxia incubator (1% O₂, 5% CO₂, and 94% N₂) for 8 h. The lower chambers were filled with DMEM containing 10% FBS. (B) 786-O cells were placed in a normoxia or hypoxia incubator (1% O₂, 5% CO₂, and 94% N₂) for 8 h. Proteins were extracted and subjected to Western blot analysis with indicated antibodies. Representative blots are shown. (C) Caki-1 cells were seeded onto Transwell inserts and subjected to Transwell migration assay in a normoxia or hypoxia incubator (1% O₂, 5% CO₂, and 94% N₂) for 8 h. The lower chambers were filled with DMEM containing 10% FBS. (D) Caki-1 cells were placed in a normoxia or hypoxia incubator (1% O₂, 5% CO₂, and 94% N₂) for 8 h. Proteins were extracted and subjected to Western blot analysis with indicated antibodies. Representative blots are shown. Bar graphs show quantitative results among groups and the value in normoxia group was defined as 100% (A–D). * p < 0.05 vs. normoxia control, n = 3.