Skip to main content
. 2019 Jun 4;20(11):2738. doi: 10.3390/ijms20112738

Figure 5.

Figure 5

Overexpression of FKBP5 reduces pAKT/AKT ratio in the dorsal hippocampus. (A) FKBP5 protein expression from DH tissue punches normalized to total protein. Calculated ratio of (B) pAKT/AKT and (C) pGSK3β/GSK3β in DH. (D) Representative Western blots for DH. Protein expression calculated for (E) FKBP5, (F) pAKT/AKT and (G) pGSK3β/GSK3β ratios in the VH. (H) Representative Western blots for VH. Tissue punches were also extracted from the AMYG to assess protein expression of (I) FKBP5 followed by (J) pAKT/AKT and (K) pGSK3β/GSK3β in this structure. (L) Representative Western blots for AMYG. Interaction of stress and genotype was analyzed by two-way ANOVA followed by Bonferroni correction when significant * = p < 0.05.; # = significant difference between stressed and non-stressed rTgFKBP5 mice. Values are represented as standard error of the mean (SEM) and protein was normalized to total protein expression. DH = dorsal hippocampus, VH= ventral hippocampus, AMYG = amygdala, ELS = early life stress.