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. 2019 Jun 3;20(11):2731. doi: 10.3390/ijms20112731

Figure 1.

Figure 1

Esculetin inhibits agonists-induced platelet aggregation in washed human platelets. (A) Chemical structure of esculetin (C9H6O4). (B) Washed human platelets (3.6 × 108 cells/mL) were preincubated with the solvent control (0.1% DMSO) or esculetin (10–100 μM) and subsequently treated with 1 μg/mL collagen, 0.01 U/mL thrombin, 1 μM U46619, and 60 μM AA to stimulate platelet aggregation. The aggregation curves in human platelets were monitored using lumi-aggregometer (Payton Associates, Scarborough, ON, Canada). ΔT/min = change in light transmission per min. (C) Concentration–response histograms of esculetin demonstrating its inhibitory activity for platelet aggregation (%). All data are presented as mean ± standard error of the mean (n = 4). * p < 0.05 and *** p < 0.001 vs. DMSO-treated group.