Figure 2.

Effect of esculetin on surface P-selectin expression, ATP release, cytotoxicity, and LDH release in human platelets. Washed platelets (3.6 × 10⁸ cells/mL) were preincubated with 0.1% DMSO, esculetin (50 and 80 µM), or FITC–P-selectin (2 µg/mL); collagen (1 μg/mL) was then added to trigger either (A) surface P-selectin expression or (B) ATP release (AU: arbitrary unit). The right panel of each figure shows the respective statistical data in (A,B). For other experiments, (C) washed platelets were pretreated with 0.1% DMSO or esculetin (100 μM) for 10 min and subsequently double washed by Tyrode’s solution; collagen (1 μg/mL) was added to activate platelet aggregation. ΔT/min = change in light transmission per min. (D) Washed platelets were preincubated with 0.1% DMSO or esculetin (50, 80, and 100 µM) for 20 min, and a 10-µL suspension of the supernatant was deposited on a Fuji Dri-Chem slide (LDH-PIII). Data are presented as mean ± standard error of the mean (n = 4). Profiles in (C) are representative of four independent experiments. *** p < 0.001 vs. resting control; ^# p < 0.05 and ^## p < 0.01 vs. DMSO-treated group.