Skip to main content
. 2019 Jun 3;20(11):2731. doi: 10.3390/ijms20112731

Figure 5.

Figure 5

Esculetin inhibits OH· formation in platelet suspensions and the closure time in human whole blood, which are estimated by the respective electron spin resonance (ESR) and PFA-100 analyses. (A) Washed human platelets was incubated with (a) Tyrode’s solution only (resting control); or with (b) 0.1% DMSO, esculetin at (c) 50 µM or (d) 80 µM, collagen (1 µg/mL) was then added for the ESR experiments, as described in the Section 4 (n = 4). Asterisk (*) indicates OH· formation. (B) Platelet plug formation induced by shear stress in whole blood was determined via recording the closure time in collagen-ADP (C-ADP)- and collagen-EPI (C-EPI)-coated membranes, as described in the Section 4. Data are presented as mean ± standard error of the mean (n = 6); p < 0.05 vs. DMSO-treated group.