Figure 6.

The in vivo activities of esculetin in thrombotic platelet plug formation in mesenteric venules, tail bleeding time, and acute pulmonary thromboembolism, in experimental mice. (A) Mice were intravenously administered 0.1% DMSO or esculetin (2.5 or 5.0 μg/kg) (all in 50 μL), and the mesenteric venules were irradiated to induce microthrombus formation (occlusion time), as described in the Section 4. Microscopic images (×400 magnification) of DMSO-treated controls and esculetin (2.5 and 5.0 μg/kg)-treated groups were recorded at 5 and 150 s after irradiation, and the platelet plug formation represents by arrows. (B) The bleeding time was measured through the transection of mice tails after 30 min of intraperitoneal administration of either 0.1% DMSO or 2.5/5.0 μg/kg esculetin. (C) For the study of acute pulmonary thrombosis, 0.1% DMSO or esculetin at various doses (2.5 and 5.0 μg/kg) was administered intraperitoneally to mice, and ADP (0.7 mg/g) was then injected through the tail veins. Data are presented as mean ± standard error of the mean (n = 8); *** p < 0.001 vs. 0.1% DMSO-treated group.