Fig. 2.
The C-terminal 7 residues of YscF are required for stable YscF expression. Y. pestis KIM5-3001, an isogenic ΔyscF strain, and the ΔyscF mutant complemented with plasmids pBAD-YscF, pBAD-YscF1-86, pBAD-YscF1-85 and pBAD-YscF1-80 were grown in the presence (+) and absence (−) of 2.5 mM CaCl2 and analysed by SDS-PAGE and immunoblotting with α-YscF antibodies. The expression (P) and secretion (S) of YopM was determined by SDS-PAGE and immunoblotting with anti-YopM (α-YopM) antisera. The location of YscF and the plasminogen activator protease-processed products of YscF present on the bacterial surface is shown by arrowheads.