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. 2018 Feb 5;164(3):338–348. doi: 10.1099/mic.0.000610

Fig. 5.

Fig. 5.

The N-terminal 2 to 15 residues of YscF are not required for stable YscF expression or secretion and synthetic amphipathic but not synthetic hydrophilic or hydrophobic N-terminal secretion signals function to promote YscF secretion. (a) Y. pestis KIM5-3001, an isogenic ΔyscF mutant, and the ΔyscF mutant carrying plasmids pBAD-YscFΔ2-5, pBAD-YscFΔ2-10, pBAD-YscFΔ2-15 and pBAD-YscFΔ2-20 were grown in the presence (+) or absence (−) of 2.5 mM CaCl2 and the cell pellets and culture supernatants were analysed by SDS-PAGE and immunoblot analysis with antibodies specific for YopN and YscF. (b) Y. pestis KIM5-3001, an isogenic ΔyscF mutant, and the ΔyscF mutant carrying plasmids pBAD-MSSSSSSS-YscF, pBAD-MSISSISI-YscF, pBAD-MSIIIIII-YscF and pBAD-MSSIISSI-YscF were similarly grown in the presence (+) or absence (−) of 2.5 mM CaCl2 and the cell pellets and culture supernatants were analysed by SDS-PAGE and immunoblot analysis with antibodies specific for YopM and YscF.