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. 2019 Jun 6;20(11):2784. doi: 10.3390/ijms20112784

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of kukoamine B (KB) on the mRNA expression levels of osteoblastic markers in MC3T3-E1 cells. After the induction of osteoblast differentiation, the cells were treated with 20 μM of KB. After treatment, the total RNA of the cells was extracted and the mRNA expression levels of Alpl (alkaline phosphatase, ALP) (A), Bglap (bone gamma-carboxyglutamate protein, Osteocalcin) (B), and Sp7 (Osterix) (C) genes were assessed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The mRNA levels of the osteoblastic markers were normalized by Gapdh (glyceraldehyde 3-phosphate dehydrogenase) mRNA expression. Control: Non-KB-treated cells. *: p < 0.05 vs. Control (Student’s t-test). All experiments were repeated three times.