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A schematic of polyacrylamide (PA) hydrogel preparation and cell seeding. (A) A schematic of PA hydrogel crosslinking and coating. (B,C) The stiff matrix induced cell spreading, and relative cell spreading area statistics are shown. Bar = 200 μm. The values are presented as the mean ± standard deviation (SD) of three independent experiments. n = 3; and ** p < 0.01.
