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. 2019 Jun 10;20(11):2831. doi: 10.3390/ijms20112831

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The soft matrix enhanced the expression of molecular markers of stemness. (A) The soft matrix increased the mRNA expression levels of the cancer stem cell markers Oct-4, Sox-2, CXCR4, CD133, and CD90, as indicated by qRT-PCR. All the results are normalized to 5.9 kPa. (B) Flow cytometry showed that the soft matrix increased the proportion of CD90+, CD133+, and CD90+CD133+ cells. (C) The soft matrix increased the number of side population (SP) cells, and the cells were stained with a negative control. The values are presented as the means ± SD of three independent experiments. n = 3; * p < 0.05; *** p < 0.001.