Table 8.
Primers used for TP53 gene.
| Exon | Primer | Primer Lenght (bp) | Exon Lenght (pb) |
Annealing Temperature (°C) |
|---|---|---|---|---|
| Exon 4 |
F-5′ TTTCACCCATCTACAGTCCC 3′ R-5′ CATTGAAGTCTCATGGAAGC 3′ |
20 20 |
279 | 54 |
| Exon 5 |
F-5′ TTCACTTGTGCCCTGACTT 3′ R-5′ AACCAGCCCTGTCCGTCTC 3′ |
19 19 |
184 | 57 |
| Exon 6 |
F-5′ CAGGGCTGGTTGCCCAGGGTCCCCC 3′ R-5′ ACTGACAACCACCCTTAACCCCTCC 3′ |
25 25 |
113 | 59 |
| Exon 7 |
F-5′ TGCTTGCCACAGGTCT 3′ R-5′ ACAGCAGGCAGTGT 3′ |
16 14 |
110 | 54 |
| Exon 8 |
F-5′ CCACCGCTTCTTGTCCTGC 3′ R-5′ CCTACTGCCTCTTTGCTTC 3′ |
19 19 |
137 | 58 |
| Exon 9 |
F-5′ AGGGTGCAGTTATGCCTCAG 3′ R-5′ ACTTGATAAGAGGTCC 3′ |
20 16 |
74 | 52 |
| Exon 10 |
F-5′ CTGAGGCACAAGAATCAC 3′ R-5′ TCCTATGGCTTTCCAACC 3′ |
18 18 |
107 | 59 |
| Exon 11 |
F-5′ GGAAGGGTCAACATCTTTTACA 3′ R–5′ TAAAAAGGGAGAAGGAGGGG 3′ |
22 20 |
1289 | 59 |