Table 9.

Primers used for PTEN gene.

Exon	Primer	Primer Lenght (bp)	Exon Lenght (pb)	Annealing Temperature (°C)
Exon 5	F-5′ ACCTGTTAAGTTTGTATGCAAC 3′ R-5′ TCTGTTTTCCAATAAATTCTC 3′	22 21	239	51
Exon 6	F-5′ CTACGACCCAGTTACCATAGCA 3′ R-5′ GGCTTCTTTAGCCCAATGAGTTG 3′	22 23	142	59
Exon 7	F-5′ TGACAGTTTGACAGTTAAAGG 3′ R-5′ CTCCCAATGAAAGTAAAGTACA 3′	21 22	167	53