Skip to main content
. 2019 May 28;20(11):2607. doi: 10.3390/ijms20112607

Figure 1.

Figure 1

Inhibition of the release of nitric oxide (NO) in lipopolysaccharide (LPS)-activated macrophages by chrysin and its derivatives. (A) Chemical structures of chrysin (CPD 1) and its derivatives (CPD 2 to CPD 10) are shown. (B) Effect of chrysin and its derivatives on the release of NO was determined by Griess reaction after LPS challenge (200 ng/mL) for 24 h in murine macrophage RAW 264.7 cells. (C) Effect of chrysin and its derivatives (CPD 1 to 10) on cell viability was analyzed by an MTT assay after 24 h incubation in RAW 264.7 cells. (D) Four additional compounds (CPD 11 to 14), which are derivatives of CPD 6 (Red Box) at atom C (5). (E,F) Effect of CPD 6 and CPD 11 to 14 on NO accumulation (E) or cell viability (F) was examined. LN, L-NAME. Data are expressed as mean ± SEM, * p < 0.05, ** p < 0.01 vs. LPS-treated group. N = 3.